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Similarly, another novel two-stage fermentation, including culture and fermentation stages, was also developed based on the analysis of conventional pH shock fermentation, achieving -PL yield and productivity increases to 32.22 g/L and 5.86 g/L/day, which were 32.3% and 36.6% higher, respectively, than those of the conventional fermentation [82]

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The inhibitor was dissolved in dimethyl sulfoxide and was kept at 20 C