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Estrogen has been shown to upregulate endogenous MOTS-c expression in preclinical models, which may partly explain why metabolic deterioration accelerates in perimenopause when estrogen falls precipitously [2]

Expression and activation of matrix metalloproteinases in wounds: modulation by the tripeptidecopper complex glycyl-Lhistidyl-L-lysine-Cu2+

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In practice, this means a researcher takes a vial of freeze-dried powder and a sterile solvent, most commonly bacteriostatic water, and combines them using a technique designed to minimize mechanical stress on the peptide