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The mixes were incubated for 15 min at room temperature and supplemented with 0.2 M E1 UBA1 and 20 M ubiquitin to initiate the reaction

This diagram serves as an effective visual tool demonstrating the energy transitions and relaxation processes that occur in molecules after the absorption of light and showing corresponding processes, such as absorption, fluorescence and delayed fluorescence (from singlet states), phosphorescence (from triplet states after intersystem crossing), and non-radiative transitions

Mixing with hCG or testosterone replacement increases the likelihood of hormone imbalance and unintended consequences