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Description

OH: Cate Schroer, Siegel, Sr

The conformational change in the sensory domain is involved in the detection of the target metabolite through the genetically encoded FRET-based sensor (a tandemly fused protein)

HPLC high performance liquid chromatography Neuroblastoma SHSY5Y (NB) cells were maintained in Dulbeco's Modified Eagle's Medium (DMEM), supplemented with 8% FCS and 8% horse serum, penicillin (25 g/ml), streptomycin (25 g/ml), 2 mM L-glutamine and 400 g/ml G418 (Gibco/BRL)

Montagnese et al

Main transport proteins and metabolic events involved in bile-flow generation and in the hepatic handling of the endogenous organic anion, bilirubin (Br) and of the model exogenous cholephilic organic anion, sulphobromophthalein (BSP)