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Indeed, erastin treatment leads to significant depletion of intracellular GSH, as detected using traditional biochemical methods and more advanced metabolomics analyses [6, 7, 28]

J Anim Sci 79:8187

Cells were broken by homogenization with 0.45 mm glass beads in the Bead-Beater (Biospect Products Bartelsville, Okla) kept on ice bath for 20 min, with cycles of 1 min treatment