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qRT-PCR was performed using SYBR Premix Ex Taq II kit (Takara) and run on LightCycler 480 Real-Time PCR System (Roche Applied Science) under the following parameters: 95C for 30 s, 40 cycles at 95C for 5 s, 60C for 30 s

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[Revised in September 2020]

10.1074/jbc.M105959200 J

Collectively, these observations highlight a convergent pathogenic mechanism in NDDs, in which mitochondrial quality control failure, through impaired mitophagy, defective protein import, and ROS-driven dysfunction, links mitochondrial damage, mtDNA-mediated inflammation, and protein aggregation [68,69,70,71,72,73]