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Using these optimized conditions (EM1 without nicotinamide, or MM + WntS + TRULI Nic, hereafter called EM2), we successfully generated expandable h-HepOrgs from 28 patients (1185 years old, 30% female) with 100% efficiency, including 5 samples from cryopreserved hepatocytes (Supplementary Table 2)

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Amino acid metabolism, mitochondrial dynamics and mitochondrial quality control An interesting aspect yet has been little explored is how mitochondrial dynamics and quality control mechanisms may communicate with amino acid metabolism

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