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ORAC Method The ORAC method was performed as described previously ( A volume of 50 L of samples to be tested or different concentrations of Trolox working solution (0.10, 0.08, 0.06, 0.04, and 0.02 mmol/L) were added to a 96-well plate, followed by the addition of 100 L of FL working solution (8.4 10 8 mol/L) and shaking for 30 s