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Gene Expression Level Analysis Total RNA of samples was isolated by using RNAprep Pure Plant kit (Polysaccharides and Polyphenolics-rich) (Tiangen, Beijing, China) and then used to synthesize the first-strand cDNA with FastKing gDNA Dispelling RT SuperMix kit (Tiangen, Beijing, China)

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Typhimurium infection strongly disturbs the steroid and eicosanoid hormone metabolism of the host