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Heneka et al., 2025) without any contribution from bone-marrow-derived monocytes to the microglial pool, at least under homeostatic conditions (Masuda et al., 2020), and healthy brain parenchyma has little macrophage and monocyte infiltration due to blood-brain barrier (BBB)

The innate immune response concerns every cell present in the CNS and is more prevalent than adaptive immunity

Cells were washed with cold PBS and then fixed with 70 % cold ethanol for 15 min on ice

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