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The Ct values were obtained from Ct values of interest genes subtracting that of loading control

Lee HJ, Jung YH, Choi GE, Kim JS, Chae CW, Lim JR, et al

The initiating factor of ALS is hypothesized to be a combination of multigenic and environmental factors [7] acting as multiple hits toward a threshold at which genetic and epigenetic changes converge into a pathologic cycle of degeneration