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These build upon similar work using ex vivo organotypic slices and 2D cancer cell co-culture [14, 163]

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Indeed, it has previously been demonstrated that in the SOD1 WT the loss of structural Zn 2+ , a common attribute of SOD1 misfolding 34 , increases accessibility of the active site to substrates other than superoxide and enhances oxidizing ability of the catalytic copper, thus facilitating ROS production 35,36