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caccae , reductions in taxa like B

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Excess biotinylating agent was quenched and removed with three washes of PBS supplemented with 0.1 M glycine and cells were homogenized using a lysis buffer (150 mM NaCl, 20 mM Tris, and 1% Triton X-100, pH 7.5) containing protease inhibitors (Roche)