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doi: 10.1006/taap.2000.8961 27 DagdagO.QuadriT

The mass tolerance for the MS/MS analysis was 20 ppm for the precursor and 0.05 Da for the fragment

Chronic sleep deprivation procedure Sleep deprivation was achieved by the disk-over-water (DOW) method as described in our previous studies (21, 22)

These effects, in conjunction with potential sleep-enhancing benefits observed in patients with dementia treated with melatonin [214], provide a compelling rationale for hypothesizing that supplementing melatonin near bedtime may augment the glymphatic systems clearance function

After three washes with PEMBAL buffer, secondary antibodies [Alexa Fluor 488-conjugated anti-rabbit IgG (Life Technologies) and/or Cy3-conjugated anti-mouse IgG (Jackson ImmunoResearch)] were added at 1/1000 dilution in PEMBAL buffer, and cells were incubated at room temperature for 3 h