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No consistent differences emerged between groups in anthropometric or metabolic parameters

Samples were harvested after 7- and 14-days incubation and extracted in lysis buffer (50 mM Tris pH7.4, 150 mM NaCl 2 and 0.01% Tween80) by bead-beating with 0.1 mm zirconia-silica beads (Sigma) in the FastPrep at 6.5 for 3 45 s with careful cooling between bursts

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In addition, one episode of severe hypoglycemia requiring intravenous glucose was reported in a semaglutide-treated patient

Following preoperative fasting guidelines helps prevent complications associated with uncontrolled glucose levels, such as hyperglycemia or hypoglycemia