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Mutagenesis of EGFP-C1STX7 was performed by GenScript

This is not ideal for several reasons, including the fact that methylphenidate may induce lipid damage in certain brain regions 27,28 and may cause abnormalities in the developing brain

After refolding, most of the protein was observed to be eluted as a monomer (TIF 578 KB) 10930_2023_10173_MOESM3_ESM.tif (download TIF Fig

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