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Mean total plaque area [in m ( To analyze the distribution of CD11b + cells, sections were fixated in ice-cold acetone for 10 min and blocked in 10% rabbit serum (Vector Laboratories, Burlingame, CA, USA)

For flow cytometry analysis, cells were harvested by trypsinization at the indicated time points after release, washed with PBS, and fixed in ice-cold 70% ethanol at 20 C overnight

Importantly, these interventions do not appear to abolish exercise-induced stress responses but rather constrain their magnitude, supporting a role for polyphenols as regulators of physiological stress balance

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