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Previous biochemical and structural studies have established that there are at least two distinct c-MET binding sites on HGF 13

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doi: 10.1016/j.bpj.2017.08.050 33 PuglisiRTramerFCarlomagnoGGandiniLPanfiliEStefaniniMet al

Of particular relevance to IGF-1 anabolic research are TSC2 (relieving inhibition of mTORC1, driving protein synthesis) and FoxO transcription factors (inhibiting their nuclear translocation, blocking expression of atrogenes like MuRF1 and atrogin-1 that drive muscle protein breakdown)