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Gene expression and integrated stress response in HepG2/C3A cells cultured in amino acid deficient medium

Analyses of biochemical parameters, including the levels of liver enzymes in the serum (ALT, AST), indicated significant damage to the livers of mice receiving D-galactose, with D-galactose inducing oxidative stress through an increase in lipid peroxidation (MDA) and decreases in the glutathione contents in brain and liver tissues

Critically, whereas ML210 and JKE-1674 cannot react covalently with purified GPX4, JKE-1777 can react with purified GPX4 and yields the same 434 Da adduct that other ML210-derived analogs generate with GPX4 only in cells (Figure 6C)