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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST

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Another device in preclinical development also uses targeted heat therapy, the RICAT (Radiometry Integrated Contrast Applied Therapy) system developed by H3Pelvic Therapy Systems, which presents as a portable, tampon-sized thermal transfer device with a specially designed probe that combines heatpipe technology with radiometric sensors to deliver and monitor precise cooling and heating cycles to the pelvic region