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Subsequently, CHAC1 has been extensively used as a ferroptosis marker, both in vitro and in vivo

Briefly, a chilled methanol extraction of the whole cells or microsome fractions was freeze-dried and resuspended in 1% acetonitrile, and then applied to a Hydrosphere C18 column (YMC) on a Prominence nano HPLC system (Shimadzu, Kyoto, Japan) in line with a 4000 QTRAP mass spectrometer (AB Sciex Instruments, Foster City, CA) equipped with Hydrosphere C18 column (YMC)

Are all metabolic syndrome components responsible for penile hemodynamics impairment in patients with erectile dysfunction