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Subsequently, bacterial suspension equivalent to 0.5 McFarland standard (1 to 2 10 8 CFU/mL) was diluted (1:150) and 100 L inoculated, totaling 200 L in each well 39

Its mechanism relies on spatiotemporal coordination among epidermal cells, immune cells, and fibroblasts through extracellular matrix (ECM) remodeling and signaling factor cascades, involving multi-dimensional molecular interaction networks such as gene expression regulation, metabolic reprogramming, and microenvironmental homeostasis

Exposure to concentrated ambient PM(2.5) (CAPM) induces intestinal disturbance via inflammation and alternation of gut Microbiome