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The reaction was then cooled to 0 C and quenched by addition of a saturated aqueous solution of ammonium chloride (3 mL)

For flow cytometric analysis of aortic endothelial cells, digestion buffer consisted of 1X PBS containing DNase I (250 U/ml) and collagenase IV (2500 U/ml, LS004212, CellSystems, Troisdorf, Germany) and aortas were digested for 40 min under agitation at 37C

Studies have demonstrated that propionate is efficiently utilized by the liver as a gluconeogenic substrate, while acetate participates in hepatic de novo lipogenesis (DNL) (56)

1B, C), which implied the edible mushrooms have a similar ergothioneine biosynthesis pathway with N

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