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J Phys Chem 117: 7843-7846

Figure 2 The two main components of the Amicon Pro purification system are the large- capacity exchange device and the Amicon Ultra 0.5 mL filter

The increase in PyroGlu could be due to three key points: (I) the catabolism of GSH through gamma-glutamyl transpeptidase, (II) and/or to a limiting factor in the second step of gamma-glutamylcysteine synthetase (III) or a limiting factor in the following step involving glutathione synthetase