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The adverse outcome pathway (AOP) framework has been rightly championed as a powerful tool for this purpose.120,162 An AOP provides a structured linkage from a molecular initiating event (MIE), through a cascade of cellular and physiological key events (KEs), to a final adverse outcome (AO) relevant to risk assessment.120 While this provides an invaluable conceptual roadmap, the classical linear structure of an AOP is a poor fit for the multi-modal nature of ENMs

Binding analyses were performed at 25 C using four different analyte concentrations: 1.25 M, 2.5 M, 5 M and 10 M (or 2.5 M, 5 M, 10 M and 20 M)

Sleep deprivation induces upregulation of the main Cl-accumulating transporter, NKCC1/SLC12A2, resulting in increased neuronal Cl accumulationa reversible process modified by the NKCC1 inhibitor bumetanide 107

Stresses 2(1):5364 Di Marzo N, Chisci E, Giovannoni R (2018) The role of hydrogen peroxide in redox-dependent signaling: homeostatic and pathological responses in mammalian cells