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The specific method is to completely mix the thawed sperm sample with ethD-1 in the kit (Purchased from Biyuntian Biological Company) at 37C for 15 min

Theoretically, the intracellular assimilation of folic acid via dihydrofolate reductase might enhance thymidylate synthetase by bypassing the methylfolate trap, thereby allowing cellular proliferation to progress temporarily

Used under medical supervision, with pharmacy-sourced compounds and proper protocols, reported side effects are generally mild

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Exp Mol Med 39 : 756768 Lowry MH, McAllister BP, Jean JC, Brown LA, Hughey RP, Cruikshank WW, Amar S, Lucey EC, Braun K, Johnson P, Wight TN, Joyce-Brady M 2008 Lung lining fluid glutathione attenuates il-13-induced asthma