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Mechanical Injection Variables Injection-related variables can influence local response, including: Shallow (intradermal) placement instead of subcutaneous Injecting too quickly Larger volume in a small surface area Repeated use of the same anatomical site Even sterile saline alone can produce a temporary raised bump if placed too superficially

Chen K, et al

The pathway works through a cascade of molecular events

Umegaki H: Neurodegeneration in diabetes mellitus

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