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Gclc is required for T cell-independent and -dependent B cell immune responses Next, we aimed to understand how the metabolic perturbations caused by B cell-specific Gclc ablation impinge on immune responses and in particular how the impaired mitochondrial metabolism affects the antibody response

For real-time PCR, RNA was extracted using RNAStat60 (Tel-Test) and treated with RQ1 DNAse (Promega, no

For GSTe4, BN-GSTe4 flies showed significantly lower mortality rates (31%, and 54%, after 12 h, and 24 h, respectively) compared to MAL-GSTe3 flies (41, and %, 73%, after 12 h, and 24 h, respectively) and control flies (48%, and 98%, 60% after 12 h, and 24 h, respectively) (Fig

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