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The SIF was a commercially available product formulated according to pharmacopeial guidelines, consisting primarily of potassium dihydrogen phosphate (KHPO), sodium hydroxide for pH adjustment, and purified water, without digestive enzymes

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Surface morphology and elemental composition were studied with a FESEM coupled with EDX [JTM-800, JEOL, JAPAN]

Moreover, overexpression of GPX4 with adeno-associated virus significantly attenuated neuronal cell death and improved mice behavior (Zhang et al., 2018)

The relative quantitative method of 2 Ct was employed to calculate the target gene expression levels, which were then analyzed in Excel