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Secondary Alexa Fluor 555 (ThermoFisher Scientific) and Alexa Fluor 647 (ThermoFisher Scientific) were used at 1/200 and 1/400 dilutions, respectively

Specifically, MYC amplification mediates the glutamine-related metabolic rewiring in breast cancers, that promotes the excessive uptake of glutamine by inducing the expression of glutamine transporters and glutamine-metabolizing enzymes (180)

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For further discussion regarding western blot protocols see, e.g., Ausubel et al, eds, 1994, Current Protocols in Molecular Biology, Vol

Docking the compound 11,426,026 into PyrG indicates that it only recognizes the ATP-binging site of PyrG, in which the phenyl ring forms - stacking with Arg223, and its nitro group forms hydrogen bonds with Ala253 and Asp252 [295]