elution reduced glutathione for gst-egfp fusion protein Transglutaminase 2 crosslinks the S-transferase tag, impeding protein–protein interactions of the fused GFP expression in E. coli.
GFP expression in E. coli. (A) Purification workflow of GST fusion Download Scientific Diagram GST Tag Definition & Data Beyond Purification: Evolving Roles of Fusion Tags in Biotechnology BioFreaks Biochemistry Blog (v.Beta): GGS LIVE Protein purification from E.coli Purification of GST Tagged Proteins ScienceDirect
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