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Increased intestinal permeability, chronic inflammation, oxidative stress, -synuclein aggregation, and neurotransmitter imbalances are some key factors that govern gastrointestinal pathology and PD progression

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There is still a gap in existing research in the field, necessitating well-designed, robust, multicenter clinical trials with sensitive endpoints and standardized protocols to facilitate the diagnosis of DPN via a simple and effective algorithm and to track the progression of disease and treatment response

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Dendritic cell maturation assay DC2.4 Cells (Thermo Fisher Scientific, Waltham, MA, USA) from C57BL/6 mice were cultured in RPMI-1640 medium supplemented with FBS (10% v/v), HEPES buffer (2.5% v/v), L-glutamine (1% v/v), NEAA (1% v/v), PSG (1% v/v) and 2-mercaptomethanol (0.00054% v/v) at 37 C in a CO 2 (5%) incubator