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EGCG is an ester formed from epigallocatechin and gallic acid, classified as a type of catechin

The pentose phosphate pathway (PPP) is the major supply of NADPH in cells and so cells were incubated with 6-amino nicotinamide, a known inhibitor of the PPP enzyme 6-phosphogluconate dehydrogenase

They proposed that melatonin upregulates antioxidant enzymes, increases distribution of cells in S-phase and decreases G0/G1 cells, reduces lipid peroxidation, all of which could explain its protective mechanism [39]

To genotype mice carrying the mutation in GLAST exon 4, PCR products (824 bp) were digested with PsiI, which gives two fragments of 604 and 220 bp in the mutant gene

J Biol Chem 270(20):12297-12304, 1995