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GFP-LC3 transient cells transfection Seven-day-old cell cultures were transfected with 500 ng GFP-LC3 expression vector using lipofectamine 3000 reagent (ThermoFisher Scientific) in Opti-MEM-reduced serum medium for 15 min at room temperature according to manufacturers instructions

How the process of singing and learning voice can be traumatizing because of abusive teachers and how fear and stage fright can develop because of it and why listening to your inner voice and knowing your "why" is crucial in the performing arts and so much more

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Mechanical stiffness promotes skin fibrosis via Piezo1-Wnt2/Wnt11-CCL24 positive feedback loop