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Afterward, the samples were vortexed and centrifuged for 2 min at 18,407 g

Several mechanisms have been proposed to explain chronic hyperglycemia-induced nerve damage, such as intracellular accumulation of sorbitol, glycation reactions, and oxidative and nitrosative stress (reviewed in 69)

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The factors that lead to depletion of glutathione includes natural ageing process, or the external stressors that include chronic exposure to chemical toxins, cadmium exposure, alcohol use, smoking, pollution, strenuous aerobic activities, dietary factors, ultraviolet (UV) radiation exposure, tissue injury as from burns, ischemia, septic shock, surgery, drugs (such as acetaminophen and adriamycin)