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doi: 10.1136/openhrt-2018-000784

2004;287(4):L774-L783

Samples were fixed in 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences) in PBS (Corning) for 30 minutes at room temperature, followed by reduction with 0.1% NaBH 4 in PBS for 10 minutes and three times of washing with PBS