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NAC dose can overdose

Darbandi M, Darbandi S, Agarwal A, Sengupta P, Durairajanayagam D, Henkel R, et al

doi: 10.1186/s40478-020-00924-7 223 DuanJCuiJYangZGuoCCaoJXiMet al

Protein Purification The thawed cell pellet was resuspended in 120 mL of buffer A [50 mM sodium phosphate (NaPi) (pH 7.5), 5% (v/v) glycerol, 200 mM sodium chloride (NaCl)], with 2 mM phenymethylsulfonylfluoride, 2 mM magnesium chloride, lysozyme (30 mg) and DNAse (10 mg) at 4 C, and disrupted by sonication (Branson Ultrasonic)