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10.1016/s0741-8329(01)00168-9 22

For YNB medium transfer experiments, fresh overnight cells of each strain were then inoculated into the filtered spent medium (SM) and aliquoted into a 96-well plate at an OD 600 of 0.0001 and grown for 72 h at 30C with shaking at 220 rpm and OD 600 measurements taken every 24 h