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A total of 4 million A375 cells were seeded in a 150-mm dish and, 24 h later, when the tumor cell population reached approximately 10 million, 30 million CD8 + primary human T cells expressing LaccID and a constitutive engineered receptor (TCR or CAR) or T cells expressing only LaccID (no TCR or CAR) were added to the culture, achieving an effector to target ratio of 3:1

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