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And it seems to pop up everywhere

10.1186/s13046-020-01724-6 75 NomuraJ.BussoN.IvesA.TsujimotoS.TamuraM.SoA.et al (2013)

This is easily one of the most exciting parts and a question we get all the time

The number of recA copies was calculated by establishing a threshold cycle (Cq) standard curve of a known number of recA gene extracted from strain B31-5A4, and burdens were normalized to 100 ng of total DNA