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After three washes with free-serum media, cells were collected, counted, and analyzed for their fluorescence intensity using a fluorescence microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) with its excitation and emission wavelengths at 488 nm and 525 nm [29, 30]

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10.1038/sj.mp.4001045 42

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Epidemeiology Global Incidence The incidence of common Guillain-Barr syndrome has been estimated to range between 0.6-4 instances per 100 000 people per year globally, although the most recent, meticulous population-based studies in Europe consistently report an incidence of 1.2-1.9 per 100 000 people 5,6