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Determination of Caspase 3 Activity Cells were plated on black, clear-bottomed collagen-coated 96-well plates at the density of 2 10 4 per well in 0.1 mL DMEM

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Following 3 h of drug treatments, 5 M of the cell-permeable, ROS-sensitive dye CellROX Deep Red (Invitrogen, Stockholm, Sweden) was added to each sample and incubated at 37 C for 30 min

[3] Nevertheless, they are part of the ATP-grasp superfamily, which consists of 21 enzymes that contain an ATP-grasp fold