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We next performed Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIPqPCR) targeting conserved PARN promoter regions using IgG or SRF antibodies in MAT2A-overexpressing MNNG/HOS and U-2OS cells, which confirmed that MAT2A enhances PARN transcription by promoting SRF binding to its promoter (Fig

It is essential to consider that most in vitro studies have evaluated the effects of phytoestrogens in an isolated manner, leaving aside the fact that a set of molecules induces the effects, and not only a single one