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In contrast, Blackett et al

2b, 3e)

C 122 , 111935 (2021)

Showed in multiple studies that combining a GHRH analog with a GHRP produces a synergistic (greater than additive) GH response because the two signals converge on somatotrophs through independent intracellular pathways [8]

Live intracellular fluorescence stainings CellROX Orange Reagent, for oxidative stress detection (C10443, Invitrogen, Thermo Fisher Scientific), FerroOrange (F374, Dojindo), and BODIPY 581/591 C11 (D3861, Invitrogen, Thermo Fisher Scientific) were used according to the manufacturers instructions