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Table 1 PCR reaction system Components Volume(L Final concentration 2 High-Fidelity PCR Master Mix (With Dye)* 25 1 Template*** x - Forward Primer10 mol/L** 2 0.4 mol/L Reverse Primer10 mol/L** 2 0.4 mol/L ddH2O Up to 50 - Note: 1) *The 1 final concentration of the mix contains 1 U/50 L of polymerase, 2 mmol/L Mg2+, and 200 mol/L dNTPs