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After ingestion (often as glucoraphanin converted to SFN by myrosinase activity in plants or gut microbiota), SFN undergoes rapid conjugation with glutathione and sequential metabolism via the mercapturic-acid pathway to produce SFNGSH, SFNCysGly, SFNCys, and SFN-N-acetylcysteine (SFNNAC), which are quantifiable in plasma and urine

Figure 3C shows that the addition of gliotoxin to the protein leads to a decrease of emission fluorescence of about 17%, and the K D value calculated with non-linear regression analysis was 307 nM (Fig

doi:10.1093/tropej/35.2.92

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doi: 10.3390/biology9120434 [DOI] [PMC free article] [PubMed] [Google Scholar] 73.Wang Q, Morris RJ, Bode AM, et al