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Description

There exists a complex interaction between the CNS and bone, with one key aspect being the neuroregulation of bone metabolism

20 mg of lyophilized small intestine was placed into a 2 mL centrifuge tube

[DOI] [PubMed] [Google Scholar] Claver JA, Quaglia AIE

The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation