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As a practical bonus, BPC-157 reportedly reduces injection site discomfort from the copper peptide

After 24 h of treatment, cell numbers were quantified by staining with 100 l of MTT solution (0.5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide in HPLM without FBS) for 90 min, then solubilized in 100 l DMSO and agitated on a plate shaker for 10 min