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At given time-points, cells were washed and lysed with 1 ml of sterile water and plated on chocolate agar enriched with PolyViteX

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The precipitates derived from each supernatant were dissolved in Tris buffer (2 mM EDTA/250 mM Tris-HCl) at pH 8.5, reduced with 0.67 M dithiothreitol, alkylated with 1.4 M iodoacetamide and digested with trypsin

This work offers a novel approach for the development of anti-influenza drugs