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The primary source of cystine in GSH synthesis is available through the cystine-glutamate antiporter xCT

The dual HPLC and mass-spec approach is the correct standard, since purity and identity are separate questions and a compound can be pure yet incorrectly identified

The newly generated pyruvate later crosses the mitochondrial inner membrane in favor of presumptive pyruvate transporters MPC (MPC1 and MPC2) and/or MCT1 (16, 49), to transform into acetyl-CoA for entering the tricarboxylic acid cycle (TCA) and therefore producing 1417 ATPs per lactate molecule for maintaining neuronal activity (26)

Doctor reviewed process with me and made things simple